Functionality¶
OMICs analysis is made easy with R tools such as “edgeR” and “limma” packages. R has serious limitations when applied to large datasets.
The First objective of edgePY is to offer an alternative free tool for such analysis.
Components¶
Input(1) -> Normalization(2) -> Analysis(3) -> Visualization/Results(4).
Input¶
Read correctly the file
A data matrix separated by tab. Of genes/proteins in lines and samples/observations in columns. Groups for the main analysis usually are defined there, or assigned to the samples.
Normalization¶
Quality -> Library -> (TMM or RLE or upperquartile or none) -> commonDispersion -> TagwiseDispersion -> Norm. Matrix
Analysis¶
Norm. Matrix -> Set the sample groups to be compared -> Statistical analysis of choice (ebayes/treat/QLF) -> DE genes list and statistics
Visualization/Results¶
DE genes list / Statistics -> Visualization ( Venn / The mean-variance relationship of log-CPM / Heatmaps / Volcano plots / Dispersion plots
More details should be added as we progress in the coding.